產(chǎn)品名稱:anti-His Tag Mouse Monoclonal antibody(鼠單克隆抗體)
產(chǎn)品型號:M1001020
產(chǎn)品特點:anti-His Tag Mouse Monoclonal antibody(鼠單克隆抗體)在Western Blotting中使用內(nèi)參其實就是在WB過程中的另外用內(nèi)參對應的抗體檢測內(nèi)參,這樣在檢測目的產(chǎn)物的同時可以檢測內(nèi)參的表達,由于內(nèi)參在各組織和細胞中的表達相對恒定,借助檢測每個樣品內(nèi)參的量就可以用于校正上樣誤差,這樣半定量的結果才更為可信。
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英文名稱 anti-His Tag Mouse Monoclonal antibody
規(guī) 格 100ul
稀釋比例 WB 1:3000 IP 1:200 IF 1:1000
濃 度 1mg/ml
保 存 Store at -20°C. Avoid freeze / thaw cycles.Stable for one year from the date of shipment.
儲存液 PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 61% Glycerol
性 狀 Lyophilized or Liquid
克隆類型 Monoclonal Antibody
宿 主 Mouse
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
說明書
Anti-His Tag Mouse Monoclonal Antibody (3C5)
Catalog No. M1001020
Size 100μl
Storage: Store at -20°C. Do not aliquot the antibody.
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His)
residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags
can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers
identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
Molecular Weight: N/A
Application: WB 1:1500 IP 1:100 IF:1:500 Optimal dilutions should be determined by the end user.
Specificity: The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag
fusion proteins.
Form: Liquid ,1mg/ml
Storage buffer: PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol
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RG001030 Anti- GFP Tag Rabbit Polyclonal Antibody
RG001040 Anti- GST Tag Rabbit Polyclonal Antibody
RG001050 Anti- cMyc Tag Rabbit Polyclonal Antibody
RG001060 Anti- Flag Tag Rabbit Polyclonal Antibody
Solarbio
Beijing Solarbio Science & Technology Co., Ltd
/82
Http://www.solarbio。。com
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